| abstract |
The present invention relates to a method for simultaneously amplifying a target nucleic acid and a signal probe using an external primer set, a DNA-RNA-DNA hybrid primer set, and a DNA-RNA-DNA hybrid signal probe, and rapidly detecting the target nucleic acid. According to the present invention, the target nucleic acid can be amplified more easily and quickly and accurately than the conventional PCR method, and the signal probe can be amplified at the same time. It is useful in molecular biology research and disease diagnosis as it is applied to detection, diagnosis of susceptibility to genetic diseases, evaluation of gene expression and various genome projects. |