| abstract |
The present invention is E. coli (E. Coli) and coumarin into the protein produced in the host cell, such as eubacteria unnatural amino acid L- (7- hydroxy-coumarin-4-yl), tRNA, and amino which may be introduced into the glycine ethyl It relates to an orthogonal pair of acyl-tRNA synthetases. The present invention includes, but is not limited to, for example, a novel orthogonal synthetase, a method for identifying and preparing the new synthetase, and a non-natural amino acid L- (7-hydroxycoumarin-4-yl) ethylglycine. Methods of producing proteins, and related translation systems. |