| abstract |
The present invention relates to a cryopreservative and a freezing method of sperm containing an amide, more specifically, a freeze preservative and a freeze-preserving method using an amide (Mamideformamide, Dimethylformamide, Urea) It is about.n n n In the present invention, instead of glycerol, which has a limit as a cryopreservative, it is toxic to living cells, and in particular, sperm having a lower molecular weight than glycerol, which has a negative effect on the quality of sperm in swine, which is more sensitive than the sperm of other mammalian animals. The application of amides (Methylformamide, Dimethylformamide, Urea), which are more effective than glycerol, while acting as a cryopreservative, can confirm the improved motility and survival rate of the crystallites. Therefore, the cryopreservative containing the amide substance of the present invention and the method of freezing sperm using the same have high value in the field of biotechnology, and in particular, may contribute to the development of the livestock industry through the preservation, breeding and maintenance of pig breeds. . |