| abstract |
As a primer set for amplifying a target region including a target region of detection in the UGT1A1 gene by gene amplification, a primer set capable of specifically amplifying the region is provided. A forward primer consisting of the nucleotide sequences of SEQ ID NO: 4 or SEQ ID NO: 81, SEQ ID NO: 21, and SEQ ID NO: 42, and a reverse primer consisting of the nucleotide sequences of SEQ ID NO: 13 or SEQ ID NO: 91, SEQ ID NO: 29, and SEQ ID NO: 48, respectively; Three sets of primers are used. By using this primer set, it is possible to amplify three target regions each containing a portion in which three types of polymorphisms (UGT1A1 * 6, UGT1A1 * 27, UGT1A1 * 28) of the UGT1A1 gene occur simultaneously in the same reaction solution. |