| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b04b7dd8f03481c3952e9e7c54387817 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y105-01003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y115-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0682 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2007-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ab30de25ee1ee7f2122e629413d8fc7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_674c9c49caa66ddb484a8da0a175f928
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11736b7b76cf316acdfead966fe7402a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0c7fb36ad2ea53afcef7776341c23e2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b50e82ffd379ed3b037be4d1d87951a9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56fbdad342b595906543a98cbb6b044f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cfbb37794d385edf38e85fafb3b131af
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ebb4d1a742fb59710c27a1c76514090a |
| publicationDate |
2008-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-20080101095-A |
| titleOfInvention |
Floating cell line expressing antisense lactic acid dehydrogenase mRNA and overexpressing MNSOD for the production of recombinant human EPO |
| abstract |
The present invention relates to a floating cell line in which antisense lactic acid dehydrogenase mRNA is expressed and MnSOD is overexpressed to increase production of recombinant human EPO, and induces antisense lactate dehydrogenase (LDH) mRNA having a nucleotide sequence of SEQ ID NO: 1. The present invention provides a recombinant cell line which is transformed by a vector containing a manganese peroxygen-antimutant enzyme MnSOD gene having a gene having a nucleotide sequence of SEQ ID NO: 2 and a human erythropoietin with high efficiency. According to the above configuration of the present invention, the production of lactic acid accumulated in the culture medium is suppressed and the antioxidant capacity of the cell line is increased, thereby prolonging the life of the cell line, thereby increasing the EPO productivity. It is also advantageous to adapt adherent cells to floating cells by culturing in serum-free medium, having the ease of scale-up for mass production for industrialization. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101271713-B1 |
| priorityDate |
2007-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |