http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20080097993-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b88552b3c4106c6767d75a2a38a47871 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-82 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-92 |
| filingDate | 2006-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9ccc5f2251d6f3f096a5ab5364cde6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fbeb384301eaf9ad8186da0728e04f6 |
| publicationDate | 2008-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20080097993-A |
| titleOfInvention | New Efficient Transformation Methods of Sunflower and Oilseeds Based on Positive Selection |
| abstract | The present invention discloses a highly improved, reproducible, stable transformation and regeneration method that makes it possible to obtain 12-15% of transgenic plants. The present invention relates to a method for selecting genetically transformed sunflower explants based on the ability to use xylose as the only carbohydrate source. Also targeted with a vector comprising a gene encoding the enzyme xylose isomerase under functional combination of the nucleic acid sequence of the xylose isomerase gene, the vector construction for incorporation of the selection marker, and the heterologous regulatory sequence. A method of transforming a host plant by Agrobacterium mediated transformation is disclosed. Also disclosed is a method of selecting putative transformants with metabolic benefit using xylose as the only carbon source after transformation with the vector. Application of the positive selection method disclosed herein observed increased regeneration efficiency, better growth and survival. The present invention alleviates the disadvantages of negative selection methods, such as the potential environmental harm caused by unwanted removal of transformed cells and the dispersion of antibiotic and herbicide resistant genes. |
| priorityDate | 2008-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 359.