| abstract |
The present invention utilizes a number of properties that have not been previously available for the purpose of purifying cardiomyocytes or newly discovered properties. Myocardial muscles derived from fetal and stem cells without genetic manipulation and without the addition of special proteins and bioactive substances An object of the present invention is to develop a method for purifying cardiomyocytes from a cell mixture containing cells with high purity and high efficiency. The inventors of the present invention, the cardiomyocytes derived from embryonic stem cells in a culture solution of low serum conditions, low sugar conditions, low nutrition conditions, low calcium conditions, weakly acidic pH conditions, lactic acid addition conditions, aspartic acid-glutamic acid addition conditions and / or pyruvic acid addition conditions By culturing, it was found that efficient and high-purity cardiomyocytes can be selected and purified. In addition, the method found by embryonic stem cells was found to be applicable to the selection and purification of fetal-derived cardiomyocytes and to the selection and purification of cardiomyocytes derived from adult stem cells. |