| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c4afb8b01aa8de7e0b24a86713b23a18 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-034
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-735 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-047
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate |
2006-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f8be19d3a2a280fca0f60d7c03adaab
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a520206b974bc8860b3e4519f947c77a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17e6fb71ae13e5525b9b08e7a092635a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82c1be977e84392bc17143ebddfdac62 |
| publicationDate |
2008-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-20080035603-A |
| titleOfInvention |
Phage display using cotranslational translocation of fusion polypeptides |
| abstract |
The present invention relates to a filamentous phage display method wherein a desired polypeptide (POI) displayed on phage particles is cotranslated and translocated through the cytoplasmic membrane of a Gram-negative bacterium, based on a signal recognition particle pathway. . This method is particularly suitable when derived from polypeptides known to be difficult to display on phage, and proteins of cDNA libraries and other combinatorial libraries, especially very fast folding, stable protein scaffolds. The invention also relates to a phage or phagemid vector comprising a gene construct encoding a fusion polypeptide comprising a POI to be displayed on the phage particle and an N-terminal signal sequence that promotes co-translational translocation based on the signal recognition particle pathway. It is about. |
| priorityDate |
2005-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |