http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20070112785-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_864bfa4a0b74643642e9a550bbe5acc5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-518 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-137 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-513 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-537 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B50-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y30-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y15-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2006-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30fbb334e8397adb087daf1e767893e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b949aa950c98cd1f9e02278b23599b94 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c090feec1c36d92ba81acd4525a1ab0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5727ed7471d09eddb4d2c493858d41a |
| publicationDate | 2007-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20070112785-A |
| titleOfInvention | Reagents, Methods, and Libraries for Bead-Based Sequencing |
| abstract | The present invention provides a method for determining nucleic acid sequences by performing a continuous cycle of double helix extension along a single strand of template. The cycle includes the steps of extension, ligation, and preferably cleavage. In some embodiments, the method uses extension probes containing phosphorothiolate chains and uses agents suitable for cleaving such chains. In some embodiments, the methods employ extension probes containing non-basic residues or damaged bases, and are suitable for cleaving the chain between nucleosides and non-basic residues and / or agents suitable for removing damaged bases from nucleic acids. Use The present invention provides a method of determining information about a sequence using at least two distinguishably labeled probe groups. In some embodiments, the method obtains less than two bits of information from each of the plurality of nucleotides of the template in each cycle. In some embodiments, sequencing reactions are performed on a template attached to beads immobilized on or on a semi-solid support. The invention also provides a set of labeled extension probes containing phosphorothiolate chain or trigger residues suitable for use in the methods of the invention. The present invention also includes performing multiple sequencing reactions on a single template by removing the starting oligonucleotide and the extended strand and performing subsequent reactions using different starting oligonucleotides. The present invention further provides an effective method for preparing a template, in particular a method for performing sequencing of many different templates simultaneously. The invention also provides a method of performing ligation and cleavage. The present invention also provides a novel library of nucleic acid fragments containing paired tags, a process for preparing microparticles to which a number of different templates (such as containing paired tags) are attached, and a method of individually sequencing templates. to provide. The present invention also provides an automated sequencing system, flow cell, image processing method, and computer-readable instructions (such as for performing an image-processing method) and / or computer-readable medium for storing sequence information. In some embodiments, the sequence information is stored in a database. |
| priorityDate | 2005-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 419.