| abstract |
The present invention relates to a placental tissue-derived pluripotent stem cell and a cell therapeutic agent containing the same, and more particularly, characterized in that the fine amnion, membrane, basal degeneration membrane or placental tissue is cultured in bFGF-containing medium and then recovered. (A) all showed positive immunological properties for CD29, CD44, CD73, CD90 and CD105, and all negative immunological properties for CD31, CD34, CD45 and HLA-DR; (b) exhibits positive immunological properties for Oct4 and SSEA4; (c) attached to plastic and grown, exhibits morphological characteristics of round- or spindle-shape, and forms a sphere in SFM medium (sphere-forming medium) to form micronized Long-term maintenance; And (d) a method for producing placental stem cells having the ability to differentiate into mesoderm, endoderm and ectoderm derived cells, and obtained placental stem cells. Pluripotent stem cells according to the present invention have the ability to differentiate into myocytes, vascular endothelial cells, osteoblasts, neurons, astrocytes, adipocytes, chondrocytes, osteoblasts, or insulin secreting pancreatic beta cells, It is effective in the treatment of muscle diseases, osteoporosis, osteoarthritis, neurological diseases, diabetes and the like, and useful for forming breast tissue. |