| abstract |
The present invention relates to a direct enzymatic reaction involving a nucleic acid molecule comprising the steps of: (a) a zwitterionic buffer and / or a non-reducing carbohydrate to overcome inhibition of enzymatic reaction of a biological sample. Performing an enzymatic reaction using a biological sample directly in a reaction mixture, wherein the nucleic acid molecules present in the biological sample are not purified prior to the enzymatic reaction; And (b) obtaining a product from the enzymatic reaction.n n n n Direct, enzymatic reaction, nucleic acid, zwitterionic buffer, non-reducing carbohydrate, PCR |