| abstract |
The present invention comprises (a) an amino acid sequence motif GDSX wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S Selecting a parent enzyme that is a transferase enzyme; (b) modifying one or more amino acids to produce variant glycolipid acyltransferases; (c) testing mutated glycolipid acyltransferases for transferase activity, optionally hydrolysis activity, on galactolipid substrate and optionally phospholipid substrate and / or optionally triglyceride substrate; (d) selecting a variant enzyme with increased activity against galactolipid compared to the parent enzyme; And optionally (e) preparing a large amount of variant enzyme. The invention also comprises an amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S A variant lipid acyltransferase enzyme characterized in that it comprises one or more amino acid modifications as compared to the sequence at any one or more amino acid residues as defined in 2, Set 4, Set 6 or Set 7. |