| abstract |
An object of the present invention is a differentiation inhibitor capable of culturing stem cells or embryonic stem cells in an undifferentiated state without using a feeder cell, a culture method using the same, a culture medium using the same, and a cell produced by culturing using the differentiation inhibitor. To provide. According to the present invention, stem cells are cultured using a low molecular weight compound, particularly a stem cell differentiation inhibitor comprising tetrahydroisoquinoline derivatives and a tetrahydroisoquinoline derivative, to cultivate stem cells in a large and safe manner in the absence of feeder cells. Provided are a method of culturing, a culture solution of stem cells containing a tetrahydroisoquinoline derivative, and a cell cultured and produced using a tetrahydroisoquinoline derivative as a differentiation inhibitor. |