http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20060030835-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1dc5d2a5ee65ccabdf0507faa521635 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2004-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b24cfd629a9f3610e5f4ae8f4fd948a |
| publicationDate | 2006-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20060030835-A |
| titleOfInvention | DNA base sequence analysis using DNA synthesis reaction |
| abstract | The invention consumes specific dNTPs while synthesizing DNA to identify nucleotide sequences, and proceeds with a separate DNA synthesis reaction that detects the consumption of specific dNTPs, while controlling the supply of specific dNTPs as a result of this exhaustion detection reaction. It is a way to find out the DNA sequence.n n n The present invention relates to a method for analyzing a DNA base sequence and to find a DNA sequence without electrophoresis. When synthesizing DNA to find the sequence in the DNA synthesis reaction for dNTP consumption, a certain amount of dNTP is supplied, and The remaining dNTP after the reaction is fed to the dNTP depletion detection DNA synthesis reaction at another point. The DNA synthesis reaction for detecting specific dNTP depletion can generate a sequence that the restriction enzyme can recognize if the specific dNTP is present. When the restriction enzyme is reacted and washed after the DNA synthesis reaction, the fluorescent dye attached to the DNA end is also washed away. Otherwise, the restriction enzyme cannot make a recognizable sequence, so it is not washed out after the restriction enzyme reaction. Therefore, a specific dye dNTP is sent to the detection DNA synthesis reaction after consumption DNA synthesis reaction to find out how much was consumed by the fluorescent dye detector. Base sequence of DNA synthesized in synthesis reaction can be determined.n n n For this, dGTP, dATP, dTTP, and dCTP are supplied to the DNA synthesis reaction for consumption in order, and the degree of consumption is confirmed in the DNA synthesis reaction for consumption detection. By measuring fluorescence in real time, the specific dNTP to be supplied is determined, and each dNTP is supplied in excess of 10% by the amount required to calculate the fluorescence and the degree of sequencing so that the sequence of the same base can be identified.n n n The most excellent method of living out to date is the use of electrophoresis, so it is difficult to identify the nucleotide sequence more than 800 fundamentally, but the present invention can be identified up to 10,000 or more according to the improvement of enzymes. Speed and accuracy can be increased by performing the same reactions simultaneously or by identifying complementary DNA strands.n n n n DNA base sequence, electrophoresis, living method, fluorescent dye, restriction enzyme, DNA synthesis reaction for dNTP consumption, DNA synthesis reaction for dNTP consumption detection |
| priorityDate | 2004-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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