| abstract |
The present invention relates to an E. coli mutant strain with increased antibiotic sensitivity, product secretion efficiency, and transformation efficiency, wherein the mutant strain inhibits the formation of biofilm, thereby inhibiting the proliferation of antibiotics, secretions, and foreign genes by the biofilm. It is characterized by being minimized. According to the present invention, in the strain selection process during the genetic modification experiment of E. coli, the desired strain can be selected with a smaller amount of antibiotics, and the strain other than the strain to be screened forms a biofilm and shows resistance to antibiotics. It is possible to prevent the deterioration of the efficiency. In addition, the diffusion efficiency of secretions can be increased to increase the yield of products secreted into the medium. In addition, the transformation efficiency can be increased to facilitate mass production of useful substances. |