| abstract |
The present invention relates to an apparatus, system or method for producing glycosylated proteins, particularly high-mannose glycosylates, in plant culture, while targeting and / or targeting proteins with ER signals. The present invention also relates to vectors and methods for producing and expressing enzymatically active high-mannose lysosomal enzymes using transgenic plant roots, particularly carrot cells. More particularly, the present invention relates to host cells, in particular transgenic suspended carrot cells, vectors and methods for expressing and producing biologically active high-mannose glucocerebrosidase (GCD) in high yield. The invention also provides compositions and methods for treating lysosomal storage diseases. |