| abstract |
A method and apparatus for performing a microfluidic analysis device are presented. Monolithic elastomeric membranes connected to an integrated air manifold allow for placement and actuation of a variety of fluid control structures, for example, dense arrays of structures for separating, transferring, merging, dividing, and storing large quantities of fluids. Make it possible. These fluid regulatory structures can be used to perform pathogen detection and analysis systems, including integrated immunoaffinity capture and analysis, eg, polymerase chain reaction (PCR) and capillary electrophoresis (CE) analysis. The analyte solution is entered into the device and supplied to a series of immunoaffinity capture matrices in a micromachining chamber containing antibodies targeted to a variety of microorganisms, such as bacteria, viruses, bacterial spores. The immunoaffinity chamber can capture, purify and concentrate the target for further analysis steps. |