| abstract |
Sites involved in the efficient production of (2R, 4R) -monatin in the amino acid sequence of wild-type D-aminotransferase having the amino acid sequence set forth in SEQ ID NO: 2 (positions 100, 180-183, 243, 244) Efficient substitution of amino acid residues at one or more positions of) to produce highly sweet (2R, 4R) -monatin from 4- (indol-3-ylmethyl) -4-hydroxy-2-oxoglutaric acid Can be modified to |