| abstract |
The present invention relates to a kit consisting of reagents capable of extracting genomic DNA from human blood and a method for extracting genomic DNA from human blood using the same, wherein tris-HCl buffer of 20 mM, pH 7.5 in uncoagulated human blood Adding a solution kit for erythrocyte lysis comprising a solution to remove erythrocytes, plasma, platelet components, and the like and to obtain leukocytes only; A second step of eluting the leukocyte nuclei by adding a leukocyte lysis solution kit consisting of 10 mM, pH 8.0 Tris-HCl buffer solution, 1.0 mM EDTA and 1.0% SDS to the obtained leukocytes; A third step of separating genomic DNA and nuclear protein from the nuclei of the white blood cells by adding a solution kit for protein precipitation consisting of a sodium acetate solution of 3M and pH 5.2 to the nuclei of eluted white blood cells; And a fourth step of recovering the genomic DNA by adding a TE buffer solution kit for genomic DNA recovery consisting of 10 mM, pH 8.0 Tris-HCl buffer solution and 1.0 mM EDTA, to recover the genomic DNA. Provide extraction methods. |