http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20040091462-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd15fea28fa5c3272dd3e41dcf3b0dd3 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2003-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8dd98668692fa2e2c9eda640a924962c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c6f7ecaaf1993514fb564891a8bc3c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5182e4e2f06b61da60a3d63a9fdb034c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3acc77e320d98f44299a9f58ac45c3b9 |
publicationDate | 2004-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-20040091462-A |
titleOfInvention | Quality Control Method for Microorganisms |
abstract | The present invention relates to a method for performing microorganism quality control by analyzing gene mutations by displaying the entire genome of a microorganism on a gel. Cutting into several fragments having a single strand of sticky ends, and, independently of this, preparing a hairpin ring structure adapter having a single strand of sticky ends that can complementarily complement both ends of the cut DNA fragment. step; Binding the genomic DNA fragments and hairpin ring structure adapters using DNA ligase; Removing the DNA fragment and the hairpin ring structure adapter not participating in the binding reaction by using an exonuclease; Amplifying the DNA fragment using a primer and a DNA polymerase that complementarily bind to a portion of the hairpin ring structure adapter; Separating the obtained amplification product on a gel; And it relates to a quality control method of the microorganism comprising the step of confirming the genetic variation of the microorganism. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112345658-A |
priorityDate | 2003-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 97.