| abstract |
The present invention detects genetic variations in target nucleic acids, detects the presence or absence of specific nucleic acids in biological samples, prepare single-chain nucleic acid probes, and detect pre-mRNA differential splicing in a target cDNA molecule or cDNA population. To compounds, kits and methods. It uses nicking agents for the amplification of single-stranded nucleic acid fragments that contain genetic variations of the target nucleic acid, have a unique sequence that correlates with a particular nucleic acid, are complementary to the nucleic acid of interest, or comprise exon-exon linkages. By detecting and / or characterizing such short single-stranded nucleic acid fragments, genetic variation of the target nucleic acid can be identified, the presence or absence of a specific nucleic acid in a sample, a single-stranded nucleic acid probe for the nucleic acid of interest or Or the presence of an exon-exon junction in a target cDNA molecule or cDNA population. |