| abstract |
The present invention is to culture a yeast cell in which a lysosomal enzyme gene is introduced into a sugar chain biosynthetic enzyme gene mutant of yeast in a medium, to obtain a lysosomal enzyme having a phosphate-containing sugar chain from the culture, and to act α-mannosidase on it. Provided is a method for producing a lysosomal enzyme having a mannose-6-phosphate-containing acidic sugar chain. Also provided is a pharmaceutical composition for treating lysosomal enzyme deficiency in humans produced by this method. By genetic engineering using the yeast according to the present invention, glycoproteins having a phosphoric acid-containing acidic sugar chain capable of functioning as marker markers for transporting lysosomes of mammalian cells such as humans can be produced in large quantities with good purity. The glycoprotein having a phosphoric acid-containing acidic sugar chain in the present invention can be used as a drug effective for treating deficiency of human lysosomal enzymes. |