abstract |
In the present invention, cytokine gene cDNA is integrated on a substrate to evaluate the response of cancer patients to radiation therapy by analyzing the time-dependent expression patterns of cytokine genes among genes obtained over time from irradiated human peripheral blood cells. It relates to a DNA chip.n n n In addition, the present invention is to collect peripheral blood cells from a human, and irradiated with the collected peripheral blood cells in a dose used for general radiation therapy, RNA is extracted from the irradiated peripheral blood cells according to the elapsed time, and the reverse RNA is extracted To generate DNA, and amplify the cytokine gene with one or more primer pairs of the DNA fragments of SEQ ID NOs: 1 and 2 or the DNA fragments of SEQ ID NOs: 3 and 4, and time-dependent expression patterns of the amplified cytokine genes are obtained. By analyzing the method of evaluating the response of cancer patients to radiation therapy. |