http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20030060632-A

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205
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filingDate 2002-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1814f33f6f6ce0ff1b5f0325b9332d23
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publicationDate 2003-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20030060632-A
titleOfInvention A method for using organomercurial metabolic gene as a selective marker of plant transformation
abstract The present invention relates to a method of using an organic mercury metabolic gene as a selective marker of plant transformation, and more particularly, a gene inserted by infecting a plant with a transgenic bacterium into which a vector into which a target gene is inserted is introduced. The present invention relates to a method for distinguishing cells, tissues and plants in which a gene of interest is inserted, and cells, tissues and plants in which a gene of interest is not inserted.n n n The present invention (1) primer conversion step for plant expression of merB gene; (2) amplification of the merB gene; (3) the gene is introduced so as to be located downstream of a promoter controlling its expression, and a method of producing an expression vector replicable in an Agrobacteria bacterium; (4) introducing the expression vector into an Agrobacteria bacterium to obtain a transformed Agrobacteria bacterium; (5) infecting the plant with the transformed Agrobacteria bacteria obtained in the above step; (6) Provides a method of using the organic mercury metabolic gene as a selection marker of plant transformation, comprising culturing the infected plant obtained above in a medium containing organic mercury.
priorityDate 2002-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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