| abstract |
The present invention provides an efficient and efficient method for empirically determining whether any genes are essential for each gene in the genome of an organism, and in the case of pathogenic organisms, whether they are required to be toxic or etiologic. The method of the present invention involves the construction of a genetic mutant in which one allele of a particular gene is inactivated while another allele is located under the conditional expression. The identification of genes essential for the development of essential genes and toxic infections provides the basis for the development of screening for novel drugs for such pathogenic organisms. The present invention also provides Candida albicans, which has proven to be an essential and powerful target for drug screening. The nucleotide sequence of the target gene can be used for various drug discovery, such as the discovery of recombinant proteins, hybridization assays, and structures of nucleic acid sequences. The use of genetically engineered cells comprising proteins encoded by the requisite genes in various screening methods, and denatured alleles of the essential genes also belong to the present invention. |