| abstract |
The present invention relates to a novel heat-resistant DNA polymerase gene isolated from an aeropyrum plant and a DNA polymerase expressed therefrom, and more particularly, to an aeropyrum perm having improved thermal stability and continuity. A novel method of replacing asparagine, which is 514th of the amino acid sequence of the aeropyrum perth DNA polymerase, isolated by the inventors using a positional mutation, which is a genetic manipulation method, using a nicked mutant DNA polymerase (m Apn DNA polymerase) The present invention relates to a method for producing a mutant DNA polymerase by expressing a mutant DNA polymerase, a gene for expressing the same, and an expression vector containing the gene in E. coli. |