| abstract |
The present invention is to provide a yeast strain expressing excessively in the yeast liver ferritin H chains and L chains, which are proteins that store iron in order to produce a yeast capable of storing excess iron.n n n The yeast strain amplifies the CYC1 terminator of S. cerevisiae by PCR into the pBlucriptll SK vector, cuts and inserts it into Sacl and Sacll, amplifies the Inu1 promoter of Kluyveromyces maxianus by PCR, and cuts into Kpnl and Sall, and inserts the pYESTrp2 vector. Obtained by inserting fragments containing the 2u origin and Trp1 genes cut from Sspl. |