http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20020059370-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2000-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20020059370-A |
| titleOfInvention | Methods and compositions for the construction and use of fusion libraries |
| abstract | The present invention provides a library of fusion nucleic acids, each comprising a nucleic acid encoding a nucleic acid modification (NAM) enzyme and a nucleic acid encoding a candidate protein. The invention also provides a library of fusion polypeptides comprising nucleic acid modification (NAM) enzymes and candidate proteins. The present invention provides a library of expression vectors comprising (i) a nucleic acid encoding a nucleic acid modification (NAM) enzyme and a nucleic acid encoding a candidate protein, and (ii) an EAS. At least two of the candidate proteins are different. Preferably, the NAM enzyme is a Rep protein. In addition, the EAS is preferably 20 or more nucleotides in length. Similarly, preferred embodiments utilize fusion nucleic acids comprising a nucleic acid encoding a presentation structure, a nucleic acid encoding a label or a nucleic acid encoding a targeting sequence. The present invention also provides a library of nucleic acid / protein (NAP) conjugates comprising a fusion polypeptide comprising a NAM enzyme and a candidate protein, respectively. The NAP conjugate also includes an expression vector comprising a nucleic acid encoding a NAM enzyme, a nucleic acid encoding a candidate protein and a fusion nucleic acid comprising an enzyme attachment sequence (EAS) recognized by the NAM enzyme. EAS and NAM enzymes are covalently linked. The invention also provides a library of host cells and a method for screening. |
| priorityDate | 1999-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 380.