abstract |
SUMMARY OF THE INVENTION An object of the present invention is to provide an α-isomaltosylglucose glycoside-forming enzyme, a method for producing the same, and a cyclic tetrasaccharide prepared using the enzyme and a saccharide containing the same. That is, specifically, an object of the present invention is an α-glucosyl transfer from a saccharide having a glucose degree of polymerization of 2 or more with α-1,4 glucosyl bond as a non-reducing terminal binding mode without substantially increasing reducing power. To promote a glycopolysaccharide having a glucose polymerization degree of 3 or more having α-1,6 glucosyl bond as a non-reducing terminal binding mode and α-1,4 glucosyl bond as a non-reducing terminal binding mode. α-isomaltosylglucose glucoproducing enzyme is established, α-isomaltosyl transfer method using the enzyme, α-isomaltosyl glucoglucose production method, α-isomaltosyl glucoglucose synthase and α- Cyclo {→ 6) -α-D-glucopyranosyl- (1 → 3) -α-D-glucopyranosyl- (1 → 6) -α-D-glucopyranosyl- using isomaltosyl transferase Preparation of Cyclic Tetrasaccharide Having a Structure of (1 → 3) -α-D-glucopyranosyl- (1 →} It solves by the method and the use of the saccharide obtained by this method. |