http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20020029476-A

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classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113
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filingDate 2000-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c317fa7ce26ccc387dbce3aa1a1e671a
publicationDate 2002-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20020029476-A
titleOfInvention Lysis buffer reagent for gene extraction by one step
abstract The present invention relates to a Lysis buffer reagent for extracting a gene in one step. More specifically, the present invention relates to a technique for extracting DNA genes in one simple step depending on the composition of the lysine buffer.n n n The prior art has consumed quite a lot of time and money because it is possible to detect the desired gene by PCR method after extracting the DNA gene through several steps from animal tissue or cells. In addition, there was a disadvantage in that only one sample should be tested at a time.n n n Thus, the present invention is a lysing buffer for extracting the gene by one step characterized in that the gene can be extracted from a part of the animal tissue or cells of the animal in a single step using a predetermined lysis buffer reagent Reagents are shown.n n n Therefore, by using the lysine buffer reagent according to the present invention, the action of the lysine buffer can be smoothly performed, and it is possible to directly use by PCR using the DNA gene extracted simply by one step in the experiment. Was done.n n n In addition, not only can the time be shortened, but the test method becomes simpler, which opens the way for experimentation in large quantities.
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