http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20020022701-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0024 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P35-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P35-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-02 |
| filingDate | 2000-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2002-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20020022701-A |
| titleOfInvention | Method of production of 7-aminodesacetoxycephalosporanic acid(7-ADCA) |
| abstract | 7-aminodesacetoxycephalosporanic acid (7-ADCA). The process of the invention is produced directly by fermentation of Acremonium chrysogenum (also called Cephalosoprium acremonium), D-amino acid oxidase (DAO) and gluta It is based on the use of desacetoxycephalosporin C (DAOC) catalyzed by the enzymatic activity of reel-7-ACA acylase (GLA). DAOC production by Acremonium chrysogenum fermentation is based on inactivation of the cefEF gene followed by expression of the cefE gene to develop the Acremonium chrysogenum ΔcefEF-cefE strain. The present invention is directed to: (I) desacetoxycephalosporin C synthase (DAOCS or expansion enzymes) and desacetylcephalosporin C synthase (DACS or hydroxylase) of the acremonium chrysogenum encoding the enzymatic activity inactivates the cefEF gene; (II) expressing a cefE gene encoding DAOCS enzymatic activity derived from a bacterium, in a transformants of acremonium chrysogenum in which the cefEF gene is inactivated; (III) develop a fermentation method of a recombinant strain producing DAOC; And (IV) enzymatic bioconversion from DAOC catalyzed by DAO and GLA enzymes to 7-ADCA, wherein DAO and GLA in Acremonium chrysogenum ΔcefEF-cefE, a strain that produces DAOC, and By expressing the gene together, or by expressing a gene encoding cephalosporin acylase in Acremonium chrysogenum ΔcefEF-cefE, it is possible to produce 7-ADCA directly by fermentation of Acremonium chrysogenum. . |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115175992-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021054545-A1 |
| priorityDate | 1999-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 308.