http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20010083442-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6efadd52e7ee7d81bbdb5499e61eb170 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H6-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-00 |
| filingDate | 2000-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d61c80793bad40000b1c62768240f68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44d7c9eddc664c6f133b9c43784cf33c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_559aec9bf7fae199a435f286d8c62cdb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0da5e95cf67a1992d0ff86697b45caf9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7970d312cb3f0f8088ae2cb6b3f09748 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d16d56dadcace41fc65001cc0f1a23c |
| publicationDate | 2001-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20010083442-A |
| titleOfInvention | The mass production by somatic embryos and culture method of water-dropwort |
| abstract | In order to produce clean buttercups in the facility throughout the year, development of economical and efficient breeding methods that can supply uniform and excellent seedlings in large quantities, regardless of season, must precede. The nutritional breeding method, which is a conventional buttercup breeding method, requires a lot of effort, is restricted by seasonality, and has difficulty in obtaining a uniform seedling. Therefore, in the meantime, the method of breeding through seeds has been developed to identify and eliminate the cause of seed germination.n n n The present invention is a "mass propagation method using somatic embryos," which is recognized worldwide as the best breeding method if practically available to develop an efficient and economical supply method of buttercup seedlings. Technology. This technique has not been attempted in buttercups by anyone other than the applicant, and much research has been conducted to make this method practical in several other crops such as carrots and celery, but there are still problems to be solved in the practical use.n n n In the present invention, when the tissues of the buttercups are cultured in a medium containing proper nutrition and plant growth regulators, and cultured in an appropriate environment, a cell mass (callus) is generated, and the somatic embryos in the mass of the cells are formed by water. A myriad of somatic embryos, which are different from the embryos of pollen and egg cells, are formed. This is based on the phenomenon of germination under certain conditions and the same vegetation as tissues from which tissues are removed.n n n The present invention relates to a method of obtaining a complete plant by inducing somatic embryos in buttercups, and a general description of the buttercup seedling formula using a rice transplanter.n n n Specific details of the present invention are the composition of plant tissue and medium effective for the formation of embryogenic callus, which is the stage of development and somatic embryo, the method for mass production of callus in a bioreactor, the composition of the medium for organic somatic embryo from embryogenic callus, Growth regulator composition for homogenization of somatic embryos, liquid culture method for enhancing the efficiency of somatic embryo development, somatic embryo development in a bioreactor, germination of somatic embryos, purifying and seedling conditions. In addition, seedling medium life, seeding density and seedling density, proper planting length, seedling and stem strength, suitable for mechanical seeding, and application of planting buttercups to the rice transplanter, and fertilization and physical stimulation during seedling period, packing conditions and planting intervals And so on.n n n In order to obtain somatic embryos, the embryogenic callus must first be aborted. The embryogenic callus, which is organic in the buttercup, is milky white and is best organic from immaturation. The most effective medium composition for Embryogenic callus organics was 2,4-D 1.0 mg / l addition to MS composition. Mass production of uniform and normal somatic embryos from the embryogenic callus is performed by physically using a two-step separation method in which embryogenic callus is passed through 40 and 60 mesh, and chemically ABA 0.05 mg / There is a method of adequately supplying 10 mg of L + PEG + 1.0 mg / L of BA for each developmental stage of the somatic embryo. Somatic embryos germinated well on urethane sponge with MS medium, and germination rate increased in well ventilated condition. The germination rate of somatic embryos was increased when they were stored at 0 ° C. for 10 days. The most effective somatic embryo was 1.0cm in germination, and low luminosity of 850 lux was adequate at the beginning of germination, but 27,000 lux intensity was required for purification.n n n In order to use the rice transplanter when planting buttercup seedlings on the open ground, lay a non-woven fabric on the bed to prevent the roots from coming out of the bed and put the ocher soil in the bed. Therefore, it is preferable to sterilize or heat-treat the medium during preparation. Seeds should be germinated by the germination rate before sowing, and further study is needed. A suitable height for transplanting is about 10cm, and during fertilization, fertilization or physical stimulation of siliceous fertilizer is required. When the pavement is settled on the rice paddy, the ground does not harden immediately after the rice paddies. Even if the rice plant grows upright, the graves are upright and do not stand up. It is good to harden after packing. The rice transplanter, which is manufactured based on rice, has a formal interval of 30cm, so if the parsley is transplanted, it may quickly cover the pavement due to creep.n n n Using this method, the maintenance of the mother's head is almost inexpensive, and somatic embryos having seed-like performance in a small space can be mass-produced continuously for a year, thereby significantly increasing the breeding efficiency of the buttercups. In addition, all processes are carried out in the cabin, so production is possible without being affected by the weather. In addition, there has been no practical use of somatic embryos so far, but this patent has a meaning that this technology is one step closer to practical use. In addition, if the buttercups are grown in the open field, it is possible to reduce the waste of time and labor, which may be the most disadvantage of the open field cultivation, thereby enabling the automated mass production of the buttercup cultivation. In addition, it will have the meaning of devising a technology that can use the rice transplanter in the formulation of leafy vegetables. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102577957-A |
| priorityDate | 2000-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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