Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_465082d534ca37c45b4010f6c8d94354 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8776 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-16 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
filingDate |
2000-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92f68e6ee5847f65b070cf2e485b2ca4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04ae6c313709ada137719980f9b945ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc616e47ae616548268770b2bf4997b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67cc1823f8603aeffb5d2ebe04aff23d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3ef283b9a65604847f6111ea29701c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd882d72475c8e9a8420ed5405a73bec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0a0ab24ba8d4cd30824716b5c5e4f50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ff26db69d2aef6bb60f5fa40340a880 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03e0011b51a7174d0b561d260ece026e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a13192e975b5d7ba88a2ddd6751d8d9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2956c91f7426dc3f1b22ca6971f60985 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5cdb713b915522130c96113f7814c7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c400318a1075332f9bbdd00b0a22dada |
publicationDate |
2001-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
KR-20010076941-A |
titleOfInvention |
Embryo from wild animal with inter-species nuclear transplantation and method for production thereof |
abstract |
Tiger production fertilized eggs production method of the present invention, (a) collecting the immature egg from the slaughtered ovary, culturing; (b) isolating and preserving the cell line from the body tissue of the tiger; (c) dissecting a portion of the zona pellucida of the mature egg prepared in step (a) and removing 10-30% of the cytoplasm to perform denuclearization; (d) transplanting the donor cells prepared in step (b) into denucleated eggs of step (c); (e) electrically fusion of the embryonated eggs implanted and adhered in step (d) to a voltage under DC conditions; (f) activating the fusion cells simultaneously with the fusion or in the medium to which the chemical is added; And (g) inspecting the activated cells after culturing in post-activation and microculture.n n n Donor nucleus cells are cells isolated from the tiger's ear, and preservation of these somatic cells can be accomplished by subculture, serum starvation or cryopreservation. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100479638-B1 |
priorityDate |
2000-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |