http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20010073991-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7e015cebec66f3872cd4aebb613462c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7519527cd6241f2583d69c879610c91f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2000-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c69ffff1d36672943a6a781513d6712f |
| publicationDate | 2001-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20010073991-A |
| titleOfInvention | A method for preparing massively RmlC protein and purified RmlC protein of Mycobacterium tuberculosis by it |
| abstract | The present invention relates to a method for producing a large amount of RmlC protein, a gene product of rmlC, one of genes for synthesizing rhamnose, an important component constituting the cell wall of Mycobacterium tuberculosis. More specifically, the present invention is an improvement of the fusion of 15 unnecessary amino acids to the previously reported Mycobacterium tuberculosis RmlC recombinant protein, transformed into a recombinant plasmid capable of producing a large amount of natural RmlC protein, the plasmid The present invention relates to a recombinant Escherichia coli, a method for preparing a Mycobacterium tuberculosis RmlC recombinant protein using the E. coli, and a method for purifying a recombinant RmlC protein prepared therefrom and a purified RmlC protein. According to the present invention, a large amount of RmlC protein expressed in a recombinant plasmid containing the rhamC biosynthetic gene rmlC, which is known as a drug development target of Mycobacterium tuberculosis, is composed of only RmlC protein in nature without fusion of unnecessary amino acids. I have it. Therefore, the recombinant plasmid of the present invention, a transformant thereof, a method for preparing and purifying a recombinant RmlC protein, and a natural rhamnose biosynthetic enzyme RmlC purified thereby are very effective for mass production of a natural rhamnose biosynthetic enzyme RmlC. It is very important and indispensable for crystal production and X-ray crystal analysis of RmlC protein, which is an essential step in drug development targeting RmlC protein. |
| priorityDate | 2000-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 247.