http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20010072538-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0a9674a20edffd107c7911d483a807f5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2000-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b2ef604881f1ac6eed27bd99b3756ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_80dae1d70dbb647ca18304ac5aa887f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90690bfb5dac5356ac3e8441352e0312 |
| publicationDate | 2001-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20010072538-A |
| titleOfInvention | A method for identifying dna mutation using microwell and kit therefor |
| abstract | The present invention relates to a method for identifying a DNA mutation using a microwell and a kit used in the method, which can inexpensively, easily and stably identify mutations such as substitution, insertion and deletion of bases in DNA. Identification method of DNA mutation using the microwell of the present invention comprises the steps of using a primer combined with biotin, and amplifying a portion of the DNA to identify the mutation by PCR method to obtain a biotin-bound base sequence; Constructing a probe consisting of a normal sequence of a DNA portion to be identified for mutation; Coupling the probe constructed in the previous step to an amine group in the microwell; Adding the biotin-bound nucleotide sequence obtained in the previous step to the probe-coupled microwell; Adding streptavidin-degrading enzyme to the microwells to bind the electrolytic enzyme to the biotin of the probe; And adding a substrate to the microwell to react with the electric streptavidin-degrading enzyme, and measuring color change or absorbance exhibited by the enzymatic reaction. According to the present invention, in any DNA mutation identification experiment, if a probe consisting of the nucleotide sequence of the DNA portion to be identified and a predetermined portion of the DNA can be amplified, it is cheaper, simpler and more stable than any conventional method. Mutations can be identified. |
| priorityDate | 1999-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.