Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_465082d534ca37c45b4010f6c8d94354 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0273 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-16 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-16 |
filingDate |
2000-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03e0011b51a7174d0b561d260ece026e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c400318a1075332f9bbdd00b0a22dada http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b720632f1c6f0b3bfbf13b63aee5ab30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d406ab838ca403aabe0d2b233bdb688c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5cdb713b915522130c96113f7814c7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc616e47ae616548268770b2bf4997b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67cc1823f8603aeffb5d2ebe04aff23d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0a0ab24ba8d4cd30824716b5c5e4f50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92f68e6ee5847f65b070cf2e485b2ca4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ff26db69d2aef6bb60f5fa40340a880 |
publicationDate |
2001-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
KR-20010069215-A |
titleOfInvention |
Clone animal reproduced from somatic cell and method for production thereof |
abstract |
The method of producing a cloned cow of the present invention, (a) collecting the immature egg from the slaughtered ovary, culturing; (b) isolating and preserving cell lines from various tissues of the bovine body; (c) dissecting a part of the zona pellucida of the mature egg prepared in step (a) and denuclearizing by removing 10-30% of the cytoplasm; (d) transplanting the donor cells prepared in step (b) into denucleated eggs of step (c); (e) electrically fusion of the embryonated eggs implanted and adhered in step (d) to a voltage under DC conditions; (f) activating the fusion cells simultaneously with the fusion and in the medium to which the chemical is added; And (g) inspecting the activated cells after culturing in post-activation and microculture.n n n The donor nucleus cell may be a cell line or cumulus cells extracted from bovine uterine perfusate, endometrium or fallopian tube, or may be a cell or fetal fibroblast isolated from bovine ears or muscles. Preservation of these somatic cells can be accomplished by subculture, serum starvation or cryopreservation. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8124832-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101025707-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100829426-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100975350-B1 |
priorityDate |
1999-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |