| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_465082d534ca37c45b4010f6c8d94354 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0273
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8771 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-16 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-16 |
| filingDate |
1999-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3ef283b9a65604847f6111ea29701c8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ff26db69d2aef6bb60f5fa40340a880
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0a0ab24ba8d4cd30824716b5c5e4f50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc616e47ae616548268770b2bf4997b6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67cc1823f8603aeffb5d2ebe04aff23d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b720632f1c6f0b3bfbf13b63aee5ab30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5cdb713b915522130c96113f7814c7a |
| publicationDate |
2001-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-20010005424-A |
| titleOfInvention |
Method of cell fusion for producing a clone animal using somatic cell |
| abstract |
The present invention comprises the steps of (a) collecting the fertilized egg or bovine somatic cells obtained by in vitro fertilization and in vitro culture of the cow and stored under washing and dispensing conditions; (b) isolating and preserving cell lines from various tissues of the bovine body; (c) dissecting a portion of the zona pellucida of the mature egg prepared in step (a) and denuclearizing 10-30% of the cytoplasm; (d) transplanting the donor cells prepared in step (b) into an egg whose nucleus has been denuclearized in step (c); (e) electrofusion of the cells transplanted and adhered in step (d) with a voltage of DC condition; (f) activating the fused cells in medium; And (g) post-activating and inspecting the activated cells, and then culturing the cells in a micro medium. In the step of the voltage under the DC condition, it is characterized in that the electrofusion is performed by adjusting the number of times of recovery x time to be within 60 s in total once or twice at 0.75 to 2.25 kv / cm. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100824218-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100829426-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9249385-B1 |
| priorityDate |
1999-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |