http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20000069110-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P35-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0024 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P35-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
| filingDate | 1998-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2000-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20000069110-A |
| titleOfInvention | ENZYMATIC PROCESS FOR THE PREPARATION OF CEPHALOSPORANIC 7$ b( g )-(4-CARBOXYBUTANAMIDE) ACID BY MEANS OF THE MODIFIED ENZYME D-AMINOACID OXIDASE OF TRIGONOPSIS VARIABILIS PRODUCED IN ESCHERICHIA COLI |
| abstract | The present invention relates to an enzymatic method for producing 7β- (4-carboxybutanamide) sephalosporonic acid using an altered enzyme D-amino acid oxidase of Trigonopsis variabilis produced in Escherichia coli. The method of expressing such an enzyme comprises (I) isolating DNA corresponding to a gene encoding enzyme D-amino acid oxidase; (II) removing the intron contained in the gene; (III) inserting the DNA fragment obtained above into a plasmid fragment that can be replicated in E. coli; (IV) fusing to the 5 ′ end of the structural region of the gene a synthetic assembler containing a nucleotide sequence encoding six histidines; (V) transforming the E. coli strain with the resulting recombinant plasmid; (VI) culturing the transformed cells of E. coli; (Iii) recovering the enzyme D-amino acid oxidase by affinity chromatography. |
| priorityDate | 1997-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 295.