http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20000066750-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5ad8562c578b061df7a2c3aee9d0e24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_559ec3960c545c6b9f01db728ad39dba |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A63B2102-32 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A63B60-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A63B53-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A63B69-36 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 1999-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2addb81bb27e148a0f66a6e9700c112f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7692ff1c37f4d5e96afdc2e41eb437d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be8a00d5e992a374d982a5f2374ed394 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2e9d1aae7cada13f3b58e61ede3d109 |
| publicationDate | 2000-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20000066750-A |
| titleOfInvention | The Method for Expressing Polypeptides in E.coli Using Carboxypeptidase Y propeptide as a Fusion partner, Recombinant Vector Used thereby and Transformant thereof |
| abstract | The present invention relates to a novel fusion partner Carboxypeptidase Y (CPY) propeptide, recombinant vector and transformants thereof for efficient expression of polypeptides in E. coli. More specifically, a cleavage site by chemical or protease at the propeptide C-terminus to obtain the desired polypeptide from the expressed fusion polypeptide with the affinity tag at the CPY propeptide N-terminus to simplify the purification of the polypeptide. It is about a fusion partner who introduced this. In expression of genes, small polypeptides are easily exposed to degradation when prokaryotic proteins are expressed in prokaryotes such as E. coli. In this regard, the present invention can stably produce small polypeptides that are easily degraded in E. coli by using the fusion partner. |
| priorityDate | 1999-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 885.