http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20000026009-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4a284d4f6c21b3563de538216cb9af4f |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K4-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K4-00 |
filingDate | 1998-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3881aa70d93ed3b5e773ee0b70cfdbdd |
publicationDate | 2000-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-20000026009-A |
titleOfInvention | Fluorescence for Fortivirus NIA Protease |
abstract | The present invention relates to fluorometry of a potyvirus NIa protease and its substrate, and specifically includes a base sequence encoding a specific amino acid sequence cleaved by the foravirus NIa protease, and C-terminal To tryptophan and 5- (2-aminoethylamino) -1-naphthalenesulfonyl [5- (2-amino ethylamino) -1-naphthalenesulfonyl, EDANSyl] group, and the N-terminus 5-dimethylaminonaphthalene-1-sulfonyl (5-dimethylaminonaphthalene-1-sulfonyl, DANSyl) and 4- (4-dimethylaminophenylazo) benzoyl [4- (4-dimethylamino phenylazo) benzoyl; Fluorescence of the NIa protease of the present invention measuring the activity of fortivirus NIa protease by measuring the increased amount of fluorescence generated from the free tryptophan group after reacting an oligopeptide bound to an absorber selected from the group Dabcyl] The measurement method can easily measure the activity of the NIa protease simply by measuring the amount of fluorescence generated as a result of the reaction, and also makes it possible to easily select an inhibitor of the NIa protease. |
priorityDate | 1998-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 183.