http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20000014094-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2df2e1189fa8605916aa5ef7b6df8f95 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 1998-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_809f2eab19487323ab8d909acb42bc9a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_889eb2bb1c353e11389043c81d45b601 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5a61f7a832f71b8d7d0a71d569d2264 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b644a634b1a7cbb26b6017064b294a54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fef692483aef076fd3c1c78f320b5f17 |
| publicationDate | 2000-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20000014094-A |
| titleOfInvention | Novel expression vector and method for producing fusion protein using same |
| abstract | The present invention relates to an expression vector capable of expressing a large amount of a fusion protein in which human epidermal growth factor and human angiogenin are fused, E. coli transformed with an electric expression vector, and a method for producing a fusion protein using the same. . By cutting the expression vector pTE4089 with restriction enzymes and inserting the T7 promoter, the inventors prepared an expression vector pTM408 comprising a T7 promoter, tetracycline resistance gene (Tc R ), and a plasmid par region. When the target protein is prepared by transforming Escherichia coli by inserting the gene to be expressed, the expression of the target gene is enhanced by the T7 promoter; ② only E. coli transformed with the expression vector of the present invention by tetracycline is selectively selected and cultured; ③ Even if passaged by the plasmid distribution region (par region), even if the expression vector of the present invention is evenly distributed in the two new cells to be produced, the target protein can be expressed in large quantities by inhibiting the production of heterologous Escherichia coli as much as possible. Confirmed. According to the present invention, by reducing the protein production yield caused by the instability of the expression vector has been raised as a problem of mass production in the system, both the method of expressing hEGF and angiogenin fusion protein in the form of inclusion bodies and secreted into E. coli cells It is possible to express the gene of interest in excellent yield. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100407792-B1 |
| priorityDate | 1998-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 296.