| abstract |
The present invention relates to a method for generating and identifying macaque antibodies against human B7, ie, human B7.1 and human B7.2, generated by screening phage display libraries, B7.1 and / or B7. 2 is directed to the generation and identification of monkey heterohybridoma using B lymphocytes from immunized monkeys. More particularly the present invention provides four monoclonal antibodies 7B6, 16C10, 7C10 and 20C9 that inhibit the B7: CD28 pathway and act as effective immunosuppressive agents. The present invention also provides light and heavy chains of three primatized antibodies derived from these monoclonal antibodies that bind B7.1 and possibly B7.2, primatized 7C10, primatized 7B6 and primatized 16C10. Amino acid sequence and complete DNA. These primatized and monkey antibodies can be used to treat certain immunosuppressive agents, such as autoimmune diseases and to inhibit organ transplant rejection. |