http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-19980080149-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
filingDate | 1998-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1998-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-19980080149-A |
titleOfInvention | In vitro transcription methods for screening natural products and other chemicals |
abstract | The present invention relates to an in vitro method for analyzing transcription of viral and cellular genes, which is automated and suitable for efficient and economical bulk screening for the purpose of finding specific chemical leader structures with selective effects on gene activity. will be.n n n Cell-free in vitro transcription methods of DNA templates comprising DNA sequences to be transcribed under the control of one or more gene-regulatory componentsn n n a) an extract from the nucleus, which is concentrated, optionally purified, and optionally supplemented, partially or completely replaced with transcription factors and / or cofactors, and one or more labeled for transcription Using nucleotides,n n n b) optionally, after the transcriptional reaction, the protein in the reaction mixture is isolated and / or degraded,n n n c) the labeled transcript is bound to a solid matrix,n n n d) removing excess labeled nucleotides,n n n e) measuring the amount of labeled transcript. |
priorityDate | 1997-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 328.