http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102490275-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-111 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 |
| filingDate | 2019-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-102490275-B1 |
| titleOfInvention | Bacterial identification method using sample bacterial RNA and kit therefor |
| abstract | An object of the present invention is to provide an identification method capable of identifying not only a limited number of species of bacteria, but many bacteria without changing reagents or conditions for identification. Furthermore, it is to provide an identification method that enables highly sensitive detection and identification with only one PCR, thereby reducing the burden of complicated operations and operators. In the present invention, a method having the following steps is used for identification of bacteria. (1) Using a first reaction system containing primers for reverse transcription to prepare cDNA containing the nucleotide sequence for identification of the bacterium to be identified, RNA extracted from the bacterium in the sample, and an enzyme having RNA-dependent DNA polymerase activity a step of performing a reverse transcription reaction and obtaining a reaction mixture containing the synthesized cDNA and the primer for reverse transcription; (2) Using a second reaction system comprising the reaction mixture, a primer pair for synthesizing double-stranded DNA containing the nucleotide sequence for identification of the bacterium to be identified, and an enzyme having DNA-dependent DNA polymerase activity, A step of performing PCR, provided that, in the second reaction system, the concentration of the primer for reverse transcription supplied from the reaction mixture is 0.08 nM or more and 20 nM or less, and (3) A step of detecting production of double-stranded DNA containing the nucleotide sequence for identification of the bacterium to be identified from the second reaction system after PCR. |
| priorityDate | 2018-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 488.