| abstract |
greater than 0 mM and less than about 500 mM arginine, greater than 0 mM and less than about 250 mM guanidine, greater than 0 mM and less than about 250 mM sodium chloride, greater than 0 mM and less than about 50 mM anionic surfactant, or 0% A wash buffer comprising more than and less than about 0.25% w/v of a non-ionic surfactant. When used during affinity chromatography purification of a protein of interest, such as an antibody, the wash buffer significantly lowers the level of host cell protein from the preparation. After affinity chromatography with the wash buffer, the protein of interest can be further purified using membrane chromatography. |