| abstract |
Provided herein are heteromultimeric constructs with reduced or silenced effector function. In an embodiment, provided is a heteromultimeric construct comprising an IgG Fc construct having a first and a second Fc polypeptide, each Fc polypeptide comprising a modified lower hinge region, wherein said first Fc the modified lower hinge region of the polypeptide comprises at least one amino acid modification, wherein the modified lower hinge region of the second Fc polypeptide comprises at least one amino acid modification different from the at least one amino acid modification of the first Fc polypeptide, and wherein the IgG Fc construct is compared to the corresponding parent IgG Fc construct. Shows reduced binding to all Fcγ receptors and Clq proteins. Also provided are methods of making such heteromultimeric constructs, and methods of reducing ADCC for an antibody construct by reducing effector function. |