http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102288264-B1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2465 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P13-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P17-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-40 |
filingDate | 2016-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-102288264-B1 |
titleOfInvention | Method for purification of recombinant human alpha-galactosidase a from material containing contaminant host cell proteins |
abstract | Disclosed is a method for producing recombinant human α-galactosidase A (rh α-Gal A) with high purity and on a large scale. The method comprises the steps of (a) culturing rh α-Gal A-producing mammalian cells in serum-free medium, (b) collecting the culture supernatant, (c) applying the culture supernatant to anion-exchange column chromatography. step, (d) hydrophobic column chromatography step, (e) column chromatography step using a material having affinity for phosphate groups as solid phase, (f) cation-exchange column chromatography step, (g) dye-philic a chemical conversion column chromatography step, and (h) a gel filtration column chromatography step. |
priorityDate | 2015-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 444.