| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-33
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-565 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5058
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-1282 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 |
| filingDate |
2018-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2021-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2021-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-102251096-B1 |
| titleOfInvention |
A cell-based method for determining an activity of botulinum toxin |
| abstract |
The present invention relates to a method of determining botulinum toxin activity. Currently, in the field of botulinum toxin titer measurement, there is a need to develop a cell-based titer assay (CBPA) to replace the mouse LD50 bioassay (mLD50). The present invention relates to an optimal CBPA assay using a monoclonal antibody having remarkably high binding affinity and specificity for N2-42F cells and SNAP25, and it is possible to measure a titer of 0.5 U or less to botulinum toxin. The CBPA assay using the cells and antibodies of the present invention is expected to be a highly reliable and reproducible cell-based titer assay for botulinum toxin. |
| priorityDate |
2018-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |