http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102237151-B1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2015-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-102237151-B1 |
titleOfInvention | Nuclease-mediated dna assembly |
abstract | A method of assembling two or more nucleic acids by generating a terminal sequence having complementarity using a sequence-specific nuclease agent (e.g., gRNA-Cas complex) and then assembling overlapping complementary sequences is disclosed herein. Is provided in. Nuclease agents (e.g., gRNA-Cas complexes) can generate double-stranded cleavage in dsDNA to generate overlapping end sequences, or create a gap on each strand to create a complementary overhanging end sequence. . Assembly using the methods described herein can assemble any nucleic acid with overlapping sequences, or use joiner oligos to assemble sequences without complementary ends. |
priorityDate | 2014-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 985.