http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102207361-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08L89-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0619 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08L89-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 |
| filingDate | 2018-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-102207361-B1 |
| titleOfInvention | A method for inducing dorsal cortical neurons from pluripotent stem cells by using tilapia collagen gel |
| abstract | The present inventors used tilapia collagen in an in vitro reconstitution assay to establish a culture substrate for regenerating the stiffness of brain tissue. By adding a crosslinking agent, the present inventors obtained a gel similar in stiffness to living brain tissue. The stiffness of the gel was 150 to 1,500 Pa, and the gel exhibited a topographical surface uniformity and high transparency. In addition, the present inventors investigated the effect of stiffness on neuronal lineage differentiation using human iPS cells, and the possibility of using the gel as a substrate for stem cell culture. Surprisingly, exposure to a gel with a stiffness of about 1,500 Pa at the beginning of neuronal induction induced dorsal cortical neurons. These findings suggest that brain-stiffness-mimetic gels can determine the final neuronal subtype. Based on these contents, it is expected that the crosslinked tilapia collagen gel will be useful for various reconstitution assays that can be used to study the role of matrix stiffness in neuronal production and neuronal function. |
| priorityDate | 2018-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 233.